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Psoralen cross-linking assay

WebThe commercially available compound EZ-link psoralen-PEG3-biotin has been used in numerous studies to crosslink DNA and double-stranded RNA for genome-wide investigations. Here we present a new probe, AP3B, which uses the psoralen derivative, 4′-aminomethyltrioxsalen, to crosslink and biotinylate nucleic acids. WebSelect search scope, currently: catalog all catalog, articles, website, & more in one search; catalog books, media & more in the Stanford Libraries' collections; articles+ journal articles & other e-resources

Frontiers rDNA Chromatin Activity Status as a Biomarker of ...

WebMar 10, 2024 · Herein, we developed a psoralen probe (PP)-based method for RNA tagging and ribonucleic-protein complex (RNP) enrichment. Isolation of both coding and … WebMar 10, 2024 · Therefore, we developed a photoreactive psoralen probe (PP) for the global enrichment of in vivo UV cross-linked RNPs (Scheme 1) and RBPs identification by mass spectrometry (MS). In this strategy, the specific isolation of RNPs is achieved by psoralen tagging of RNA upon 365 nm UV irradiation. play it again sports stores near me https://sexycrushes.com

Detecting RNA-RNA interactions using psoralen derivatives

WebPsoralen Crosslinking. Ligation of puromycin containing DNA linker to the 3’ end of mRNA using Moore-Sharp splinted ligation is a crucial step in preparation of fusible mRNA template. The efficiency of this ligation reaction, however, depends notably on the sequences of the mRNA. When long mRNA is used, the yield of ligated product is so low ... WebFig. S2 TFO-directed Psoralen Cross-linking. 4,5´,8-trimethyl psoralen (i) can be attached to a triplex-forming oligonucleotide (TFO) in such a way as to allow the 3,4 and 4´,5´-double bonds to ... Electrophoretic mobility shift assay of the cross-linked products generated from the DX-A C tile in the absence and presence of pso-TFO. The WebJun 22, 2015 · Psoralen cross-linking and Southern were performed as described earlier with some modifications (46, 47). Briefly, cells were either untreated (control) or treated … prime hoerbuch

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Psoralen cross-linking assay

Trimethylpsoralen - an overview ScienceDirect Topics

WebPsoralen molecules intercalate into DNA, similar to PYO, and upon irradiation with UVA light (365nm) crosslinking reactions are induced. The idea being that psoralen interstrand … WebOct 5, 1984 · Psoralen-crosslinking of DNA as a probe for the structure of active nucleolar chromatin Trimethylpsoralen was used to crosslink the extrachromosomal ribosomal …

Psoralen cross-linking assay

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WebSep 2, 2014 · Psoralens are tricyclic compounds that intercalate into double-stranded DNA or RNA and, on irradiation with long-wavelength (365-nm) UV light, covalently link pyrimidines on adjacent strands. More rarely, psoralen cross-links can be observed at the ends of helices (i.e., double-stranded-single-stran … WebAfter incubation, psoralen was added to the reaction and the mixture was irradiated for 10 min at 4C to cross-link double-stranded RNA formed between U1 snRNA and WT-72. …

WebPsoralen cross-linking has also been used to quantify cruciform formation in DNA, 226 assay for Z-DNA, 227,228 measure the level of supercoiling in vivo, 227,229 and probe DNA–protein interactions. 230,231 Moreover, monitoring the ratio of the two major diastereomeric adducts formed upon psoralen monoalkylation of thymine has been used … WebPsoralens can be site-specifically attached to DNA at pyrimidines (preferentially at 5′-TpA sites), and their absorption bands extend well …

WebApr 9, 1991 · The DNA-psoralen crosslink induced precipitating antibodies in rabbits with a titer of 1:102,400 by direct binding ELISA. The antiserum showed considerable binding with Z-DNA and calf thymus DNA brominated under high salt concentration which has been shown to attain Z-/analogous conformation. Inhibi … WebPsoralen, or derivatives of 9-methoxy-7 H-furo [3,2-g]chromen-7-one tricyclic ring structures, are used as photoreactive groups in crosslinkers, biotinylation compounds, and nucleic …

Web【翻译】 描述了处理对所选抗原肽具有特异性的灭活人工抗原提呈细胞(AAPCs)和人工抗原提呈细胞的方法，包括它们在包括 ...

Web5. A method as defined in claim 4, wherein said activated T lymphocytes are cytotoxic toward target cells expressing the peptide, and the peptide is selected from the group consis prime hlthcare svc gadsdWebGrass JA, Hei DJ, Metchette K, Cimino GD, Wiesehahn GP, Corash L, Lin L (1998) Inactivation of leukocytes in platelet concentrates by photochemical treatment with psoralen plus UVA. Blood 91(6):2180–2188 Sastry SS, Ross BM, P’Arraga A (1997) Cross-linking of DNA-binding proteins to DNA with psoralen and psoralen furan-side monoadducts. prime holdingWebPsoralen molecules intercalate into DNA, similar to PYO, and upon irradiation with UVA light (365nm) crosslinking reactions are induced. The idea being that psoralen interstrand crosslinks would break pi bonds in the affected nucleotides, inhibiting charge transport. prime holding lursoft