Webthat the 10x buffer be kept at 4°C for 1-2 weeks. For longer periods of time, buffer should be stored at -20°C. Aliquoting of 10x buffer is recommended if many small experiments are to be performed. 2. Thaw 10x buffer at 24-30°C, mixing end-over-end. 3. Dilute 10X RIPA Buffer to a 1X solution using ddH 2 O. http://wolfson.huji.ac.il/purification/PDF/affinity/PIERCE_PierceProteinLAgarose.pdf
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